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INDICADORES BIBLIOMÉTRICOS SOBRE O TEMA “DENGUE” E “DENGUE INFANTIL” NA BASE SCOPUS
(2021-10-20) Eliane Cristina Casimiro Alves Dias
Dengue is the most important arbovirus in the world and one of the main
health challenges in Brazil. The scenario of hyperendemicity, with epidemics of great
magnitude and an increase in severe forms of the disease has also led to an
increase in cases in special groups, such as children in whom the clinical
presentation of the disease is nonspecific and can present the severe form of dengue
as the first manifestation without the foreshadowings of warning signs, as in adults.
OBJECTIVE: The objective of this work is to provide an overview of the main
bibliometric indicators on the themes “dengue” and “children's dengue”. METHODS:
A bibliometric search was carried out in the Scopus database, in July 2020, using the
themes “dengue” and “children's dengue” to identify the main authors, institutions,
journals, languages used and areas that most publish on the topic. RESULTS: The
worldwide growth rate in the number of publications on the topic "dengue" and
"infantile dengue" show, on average, respectively 12.9% and 11.6% of growth per
year, therefore higher than the average growth of articles in the Scopus database,
which was 5.3% per year. The United States was the country with the highest
number of publications on the “dengue” theme, followed by India and Brazil. In terms
of “dengue for children”, the United States remained in the ranking of publications,
followed by India, Thailand and then Brazil, where Fiocruz is the main publication
institution for “dengue” and children's dengue”, followed by USP, UFRJ and UFMG.
In the world, Brazilian and US institutions are among the main institutions with the
most publications on the topic of “dengue” and “children's dengue”, followed by
European institutions. The areas that most studied the subject are “medicine”,
“immunology and microbiology” and “biochemistry and genetics”. It was also possible
to know the main Brazilian and world authors, as well as the periodicals in which
Brazilians and other authors publish the most on the subject. CONCLUSIONS: It can
be concluded that the theme “dengue” and “infantile dengue” have great relevance in
scientific research, having seen the impact on public health in Brazil and in the world
and represent a significant portion of morbidity and mortality in the pediatric age
group nowadays.
Falando com bebês: da detecção de sinais de risco para Autismo à intervenção precoce
(2018-03-09) Odila Maria Ferreira de Carvalho Mansur
The autism spectrum disorder (ASD) cases are characterized by qualitative
impairments in social and communication behavior and a restricted range of activities
moreover, restrict interests. On the other hand, there are needs for intervention together with
the scarcity of licensed professionals to do the intervention in children with ASD. The
objective of this work was to examine the effects of a parent/caregivers training program in
their children‟s outcome. Participated in this study three children diagnosed or suspected ASD
with 08, 16 and 18 moths-old and their caregivers. That research was quasi-experimental,
within-subjects, AB-based therapy – baseline, treatment, and follow-up. Moreover, effect size
measures for single-case designs was check with a non-overlapping index, TAU-U. The
interventions were made at Interdisciplinary ambulatory of Plantadores de Cana Hospital,
Campos dos Goytacazes city, in Rio de Janeiro state. The caregivers and their children were
evaluated by 40 minutes sessions once a week for a year; their performance was reviewed in
each session. There was an individualized developmental model program for each child. The
playful atmosphere was a priority, aiming to set up a social interaction between children and
their caregivers, as well as higher participation rates. The subject of study, Izabel has the best
answer to intervention; only she has an improvement in the interaction initiatives. She also
has advanced in body language, and facial expressions answer rates. Ana, the second child,
has improved in body language and language skills. The third child, Márcia had a statistically
significant increase of mixed (face and body language) answer rates. No statistically
significant changes were found in others variables. However, the treatment makes a positive
and noticeable clinical improvement in children. For example, Izabel show elevated face and
total expressions answer rates, and Márcia improved her body language initiative. Regarding
the caregivers, the Márcia‟ caregivers was highlighted with the increase use frequencies of
two strategies: call the child for her name and play with the child by touch, stimulating a
smile answer. The Ana‟ and Izabel‟ caregivers increase use frequencies only on strategy: use
children‟s songs and make a funny or complimentary comment for the child to play again.
The effect of the early intervention program in the performance of the research subjects was
evaluated. We conclude that the intervention program for the caregivers influenced, in a
statistically significant way, in the frequency of shifts and modality of responses in children.
From the data, it can be concluded that the caregiver intervention, guided by a professional,
can be effective and appropriate to the Brazilian socioeconomic context, which government
programs and qualified professionals lack to conduct interventions in the early autism
treatment.
Therapeutic strategies carried out in cases of a metronidazole-re sistant Trichomonas vaginalis infection: a literature review
(2023-12-24) Yasmim de Souza Leite; Laís Verdan Dib
Trichomoniasis is the most prevalent non-viral sexually transmitted infection worldwide,
caused by Trichomonasvaginalis. This parasitic infection primarily affects women, who
may experience clinical manifestations such as leukorrhea, unpleasant vaginal odor, vulvar
itching, and a strawberry cervix. While metronidazole is the drug of choice for treating
this infection, strains of T. vaginalis resistant to this medication have been increasingly
reported globally. In this context, the objective of this study was to conduct a literature
review of reported case studies involving alternative therapeutic regimens used for
metronidazole-resistant T. vaginalis strains, published up to the year 2019.The databases
used were PubMed and the Scientific Electronic Library Online (SCIELO). Scientific articles
that were fully available online and provided detailed information on alternative treatment
approaches for metronidazole-resistant T. vaginalis infections were included. Systematic
reviews, master’s dissertations, and doctoral theses published up to 2019 were excluded.
Results: A comprehensive analysis of 16 scientific articles was conducted, yielding 23 clinical
cases. Regarding therapeutic regimens, tinidazole was the most commonly used drug for
cases of metronidazole-resistant strains, followed by paromomycin and iodopovidone.
No standardized therapeutic guidelines were observed among healthcare professionals
in cases of metronidazole-resistant T. vaginalis infections based on the analyzed reports.
Esquemas terapêuticos realizados em casos de infecção por Tri chomonas vaginalis resistentes a metronidazol: uma revisão bi bliográfica
(2024-12-23) Yasmim de Souza Leite; Laís Verdan Dib
Trichomoniasis is the most prevalent non-viral sexually transmitted infection worldwide,
caused by Trichomonasvaginalis. This parasitic infection primarily affects women, who
may experience clinical manifestations such as leukorrhea, unpleasant vaginal odor, vulvar
itching, and a cervix resembling a strawberry. While metronidazole is the drug of choice
for treating this infection, strains of T. vaginalis resistant to this medication have been
increasingly reported globally. In this context, the objective of this study was to conduct a literature review of reported case studies involving alternative therapeutic regimens used
for metronidazole-resistant T. vaginalis strains, published up to the year 2019.The databases
used were PubMed and the Scientific Electronic Library Online (SCIELO). Scientific articles
that were fully available online and provided detailed information on alternative treatment
approaches for metronidazole-resistant T. vaginalis infections were included. Systematic
reviews, master’s dissertations, and doctoral theses published up to 2019 were excluded.
Results: A comprehensive analysis of 16 scientif ic articles was conducted, yielding 23 clinical
cases. Regarding therapeutic regimens, tinidazole was the most commonly used drug for
cases of metronidazole-resistant strains, followed by paromomycin and iodopovidone.
No standardized therapeutic guidelines were observed among healthcare professionals
in cases of metronidazole-resistant T. vaginalis infections based on the analyzed reports.
IDENTIFICAÇÃO DE MUTAÇÃO INÉDITA c.21G>A (W7X) NO GENE IL12RB1 EM PACIENTES COM DOENÇA MENDELIANA DE SUSCETIBILIDADE ÀS MICOBACTERIOSES NO NORTE FLUMINENSE
(2016-06-01) THAIS LOUVAIN DE SOUZA
The adverse reaction of BCG vaccine affects children with poor cellular immune
response. This deficiency is triggered by an absence of lymphocytes and macrophages,
inability to produce O2- or compromised via IL-12-IFN-γ. The decreased production of
IFN-γ is one of the causes of Mendelian Susceptibility to Mycobacterial Disease
(MSMD). The objective of this research was to investigate the cell populations in
peripheral blood (PBMC) of children with episodes of adverse reaction to the vaccine
BCG, and the expression of proteins involved in IL-12/IFN-γ axis. Methods: The study
included nine children of North Fluminense region of Rio de Janeiro State with adverse
reaction to the vaccine BCG. The expression levels of cellular markers for T
lymphocytes (CD45+ CD3+ CD4+ CD8+ cells), B-lymphocytes (CD19+), NK cells
(CD16+ CD56+) and IL-12Rβ1 (CD212+) and IFNGR1 (CD119+) receptors were
quantified by flow cytometry. The production of IFN-γ and IL17-A cytokine in the
supernatants of growing blood cells stimulated or not with phytohemagglutinin (PHA)
or BCG lysate was assessed by quantitative flow cytometry using microspheres. For
identification of pathogenic mutations we used Sanger sequencing, single base
sequencing and PCR-RFLP of c.21G mutation> A (rs150172855) in venous blood DNA
collected from children with adverse reactions to BCG history and their parents.
Results: Four children were diagnosed with MSMD. Two brothers and a third boy were
homozygous for the c.21G> A mutation in the IL12RB1 gene, and their parents were
heterozygous. The fourth boy died without collecting biological sample, but the parents
were heterozygous for the same mutation. There was a significant difference between
the ex vivo levels of expression of IL-12Rβ1 in cells from three patients with MSMD
and from four without MSMD when compared with healthy control. PBMC of patients
with MSMD produced less IL-12Rβ1 after stimulation with PHA when compared with
healthy control subjects or subjects without MSMD. Patients without MSMD and with
MSMD produced less IFN-γ when PBMC upon stimulation with PHA when compared
with healthy control subjects. BCG-stimulated PBMC of control subjects produced
more IFN-γ than those from MSMD s or without MSMD. In addition, patients without
MSMD produced more IFN-γ than in MSMD patients. There was no difference between
the levels of CD4 +, CD8 +, B and NK cells between the three groups. Discussion:
Patients with history of adverse reaction to the BCG vaccine produce lower amounts of
IFN-γ. However, patients with MSMD showed lower expression of IFN-γ and IL
X
12Rβ1 versus patients without MSMD or controls. Thus, the phenotype observed IL
12Rβ1 reduction and IFN-γ reaffirms the diagnostic condition. The homozygous allele
c.21G> triggers primary immunodeficiency deficiency of IL-12Rβ1, but low levels of
the protein are found on the surface of lymphocytes of affected patients. This is the first
report of an IL12RB1 stop-codon mutation with paradoxical cell-surface expression of
the receptor. According to in silico analysis, the mutation has a potentially harmful
effect on RNA splicing. All carriers of the mutation c.21G>A share the extended
CACCAGTCCGG haplotype (positive strand), featuring a founder effect. Conclusion:
We identified the novel c.21G> A (W7X) signal-peptide stop mutation in IL12RB1 in
four patients with MSMD in the Northern State of Rio de Janeiro. In homozygous
carriers, the mutation causes complete IL-12Rβ1 deficiency with paradoxical cell
surface protein expression in stimulated lymphocytes. Quantification of IFN-γ and IL
12Rβ1 was extremely important in the diagnosis of MSMD in this cohort of children
with episodes of adverse reaction to the BCG vaccine. Early diagnosis of MSMD
helped determining the preventive actions in order to reduce mortality and morbidity in
these patients.